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. 2011 Sep 1;3(5):250–258. doi: 10.4161/isl.3.5.16900

Figure 5.

Figure 5

Apoptosis protection induced by hPL-A was blocked by the inhibition of PI3K, p38 and MAPKs pathways, in insulinoma βTC1 cell line. The insulinoma β-TC1 cells were serum deprived (SD) for 48 h without BSA and stimulated with 500 ng/ml hPL-A and 50 nM LY294002 (LY) or 500 nM SB203580 (SB), or 200 nM PD98059 (PD) or 500 nM H89. After 48 h, both attached and detached cells were harvested and analyzed by a FACSCalibur, using the Annexin-V/Propidium Iodide apoptosis kit (Sigma-Aldrich), following the manufacturer's instruction. In (A) typical dot-plots are shown: early apoptotic cells were positive for Annexin-V-FITC only (box). For CTRL sample (not shown) we found that cells stained by Annexin-V-FITC were about 15%. The percentages of apoptotic cells are showed in (B); *p < 0.05; #p < 0.05 vs. SD/hPL (n = 4). One representative dot plot of four different experiments is visualized.