Figure 6.

hPL-A increases PDX-1 expression and improves Glucose induced insulin secretion (GIIS) in cultured human islets. Human islets were cultured in DMEM low glucose (5 mM) and 500 ng/ml of hPL-A was added every two days. After 2, 4, 8 and 14 days of treatment, cells and culture media were harvested and analyzed. We found (A) a dramatic increment in the intracellular expression of PDX-1, assayed by FACS; *p < 0.050 and **p < 0.010 (n = 4). Consistently (B) we found, by an RIA kit, that hPL-A improves insulin secretion after glucose (25 mM) challenge; *p < 0.050 and **p < 0.010 (n = 4). Data represent four different experiment with islets from four different donors.