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. 2011 Mar 11;13(2):R29. doi: 10.1186/bcr2848

Figure 4.

Figure 4

Effect of fulvestrant on heregulin β1 (HRGβ1) signalling in BT474 and MDAMB361 cells. (A) Western blots showing the effect of increasing concentrations of heregulin β1 (HRGβ1) (0.1 to 10 ng/ml for five minutes) on total and phosphorylated ErbB3, ErbB4, AKT and extracellular signal-regulated kinases 1 and 2 (ERK1/2) protein expression in BT474 and MDAMB361 cells, maintained for 7 days in the presence of either fulvestrant (100 nM) of vehicle control (ethanol). (B) Graphs showing cyclin D1 protein expression in BT474 and MDAMB361 cells maintained for seven days in the presence of either fulvestrant or vehicle control and subsequently exposed to either HRGβ1 or vehicle control for five minutes. β-actin was used as a loading control. (C) Western blots showing the effect of HRGβ1 (10 ng/ml) on the growth of BT474 and MDAMB361 cells maintained for seven days in the presence of either fulvestrant (100 nM) or vehicle control (ethanol). The results are expressed as means ± SEM of triplicate wells and are representative of three separate experiments. *P < 0.05 versus control, ***P < 0.001 versus control, †††P < 0.001 versus fulvestrant.