Figure 4.

Morphological features of autophagy in CTet-treated MDA-MB-231 cells. Autophagy induction in MDA-MB-231 cells following exposure to CTet was detected by recruitment of LC3b protein to autophagosomes (a) and fusion between autophagosomes and lysosomes in terms of co-localization of LC3b and the lysosome marker LAMP2a (b). CTet, indole-3-carbinol cyclic tetrameric derivative; CTR, control; LAMP2a, lysosome-associated membrane protein type 2a; LC3b, microtubule-associated protein 1 light chain 3.