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. 2011 Nov 1;25(21):2278–2290. doi: 10.1101/gad.171645.111

Figure 1.

Figure 1.

An active Bax mutant displays a holin-like bacterial lysis activity. (A) Diagram of Bax and miniBax. (B) Behavior of miniBax, Bax, and S105 in the pET system. BL21(DE3)pLysS E. coli transformed with the pET24b vector or pET24b constructs expressing F-miniBax, F-Bax, or S105-Flag were grown to A550 ∼0.3. Following the addition of IPTG (0.3 mM), A550 was measured at 5-min intervals. After 1 h, the cultures were harvested and subjected to SDS-PAGE, followed by Western blot with an α-Flag antibody.