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. 2011 Nov 1;25(21):2278–2290. doi: 10.1101/gad.171645.111

Figure 3.

Figure 3.

Dependence of the bacterial lysis activity of miniBax on homo-oligomerization. (A) Lysis phenotypes of miniBax and its two BH3 mutants, miniBaxL63E and miniBaxL63P, in Δ(SR) cells. The expression of these proteins was detected by Western blot as carried out in Figure 2B. (B) Homo-oligomerization of miniBax and its mutants. BMH cross-linking was carried out as described in the Materials and Methods, followed by SDS-PAGE and Western blot analysis with an α-Flag antibody. The asterisk (*) stands for nonspecific protein.