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. 2011 Nov 1;25(21):2278–2290. doi: 10.1101/gad.171645.111

Figure 4.

Figure 4.

Lysis of Δ(SR) cells by active mutants of Bax or Bak, but not by any other Bcl-2 family protein. (A) Diagram for the replacement of the first 92 amino acids of S105 by Bcl-2 family proteins in the S105/R lysis cassette in pS105. (B) Lysis curves for the BH3-only proteins were measured as in Figure 2B. (C) Expression of the indicated BH3-only proteins in Δ(SR) cells. Protein samples from cells expressing F-Bid, F-BimEL, F-Bmf, and F-Bik were diluted 1:10. Protein samples from F-Bnip3 and F-Noxa were diluted 1:5. The asterisk (*) stands for nonspecific protein. (D) Lysis phenotypes of the Bax/Bak and their mutants in Δ(SR) cells. (E) Expression of Bax/Bak and their mutants. Due to low expression, the protein sample from F-BakΔH1-expressing cells was concentrated fourfold. The asterisk (*) stands for nonspecific protein. Diagrams of miniBax, miniBak, and BakΔH1 are shown at the bottom. (F) Lysis phenotypes of anti-apoptotic Bcl-2 family proteins. (G) Expression of the indicated anti-apoptotic Bcl-2 family proteins. Protein samples from cells expressing these proteins were diluted 1:20 as compared with F-miniBax. A diagram of a truncation mutant of Bcl-xL, “miniBcl-xL,” is shown at the bottom.