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. 2011 Nov 1;25(21):2278–2290. doi: 10.1101/gad.171645.111

Figure 5.

Figure 5.

Replacement of the holin gene by functional Bax/Bak mutants generates viable chimeric λ phages. (A) Diagrams of Bax and Bak mutants. These mutants were cloned into the pS105 plasmid as shown in Figure 2A to replace S105. (B) Lysis phenotypes of the indicated Bax/Bak mutants. Expression of the indicated Bax and Bak mutants in Δ(SR) cells was detected by Western blot with an α-Flag antibody as carried out in Figure 2B. (C) Plaque formation by Bax/Bak mutants. At 75 min, the cultures were centrifuged at 22,000g for 10 min, and the supernatant was assayed for plaque formation using the MC4100ΔtonA as indicator cells as described in the Materials and Methods.