Figure 4.

Clock gene expression in control and surgical ICU patients PBL. PBL were obtained from four control subjects that received a placebo infusion of physiologic saline and from three ICU patients. The expression of Bmal1, Clock, Cry1, Cry2, Per3 and Rorα were determined by qPCR. (a) Shown are the mean fold change in gene expression observed in PBL obtained from four control subjects and three ICU patients. Error bars are SEM. Two blood samples, referred to as first and second blood draw, were obtained from each patient at a one-week interval. (b-d) show the fold change in Bmal1, Clock, Cry1, Cry2, Per3 and Rorα expression for each of the patients represented in panel A.