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Gene expression of FHL in various tissues. Identical amounts of total RNA from the skin (1), gill (2), esophagus (3), stomach (4), intestine (5), liver (6), kidney (7), heart (8), spleen (9), ovary (10) and muscle (11) were isolated and reverse-transcribed to produce cDNA. PCR amplification was performed with specific primer-3 and -4 to amplify a partial sequence of FHL (A). Corresponding β-actin bands for these samples are shown in (B). M, marker; N, negative control.
