Figure 2. Line scans and corresponding Fourier analysis from wildtype (A,B) and 3dpf sapje (C,D). Left-hand plots (A,C) show the raw individual line scans for each fish used. Right-hand graphs (B,D) show the Fourier transforms of the data. Wildtype fish have a single frequency of 10/mm representing the actual number of somites per mm in these fish. sapje fish, whilst showing an underlying frequency of 10/mm, due to the disruption to the birefringence caused by the lack of dystrophin, also exhibit several other smaller frequency modes. As is clear to the eye, pixel power of wildtype and mutant fish populations are significantly different. At 4 cycles/mm, WT: 748 ± 536 and mutant: 36504 ± 26159 (mean ± SD, p = 0.029; one-tail ANOVA).