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. Author manuscript; available in PMC: 2011 Nov 17.
Published in final edited form as: Comb Chem High Throughput Screen. 2008 May;11(4):283–293. doi: 10.2174/138620708784246022

Table 2.

Screening results in ER Binding, ERE-luciferase, and Alkaline Phosphatase Assays.

Plant or sample name Extract Type 1 Binding ER alpha 2,3 ERE-luc ER alpha 3,4 ALP Estrogenic 3,4 ALP Antiestrogenic 3,4 SRB Cytotoxicity 1 Binding ER beta 2, 3 ERE-luc ER beta
17 β estradiol Control 95 ± 1 21 ± 7 100 ± 10 −10 ± 8 4 ± 2 90 ± 1 7 ± 3
4-hydroxytamoxifen Control 96 ± 1 1 ± 0.1 0 ± 5 94 ± 1 2 ± 1 94 ± 1 1 ± 0.2
DMSO Control 0 1 ± 0.2 1 ± 2 0 ± 5 1 ± 2 0 1 ± 0.2
Alisma plantago-aquatica Petroleum ether 31 ± 5 0.9 ± 0.4 −7.3 ± 4.2 −1.5 ± 13.3 10.7 ± 16.2 51 ± 7 2.3 ± 1.9
Angelica sinensis Petroleum ether 581 ± 10 9.7 ± 3.5 −4.7 ± 6.2 48.7 ± 9.5 16.4 ± 13.6 99 ± 9 1.1 ± 0.4
Chloroform 19 ± 9 5.9 ± 3 12.3 ± 4.7 19.4 ± 9.8 30.6 ± 8 46 ± 6 0.8 ± 0.5
Butanol 4 ± 3 9.5 ± 2.2 12.4 ± 8.2 −16.3 ± 4.5 3.3 ± 15.2 0 ± 2 1.2 ± 0.2
Asclepias tuberosa Petroleum ether 69 ± 7 1.1 ± 0.4 −17.9 ± 15.5 −9.1 ± 21 −19.9 ± 9.8 85 ± 5 1.1 ± 0.8
Dichloromethane 16 ± 9 4.1 ± 3.1 −26.5 ± 4.5 114.6 ± 6.7 106.1 ± 5.3 32 ± 10 10.7 ± 0.
75% ethanol 0 ± 2 3.9 ± 4.3 −10.3 ± 8.9 110.1 ± 8.5 99.1 ± 3.6 5 ± 8 1.3 ± 1.1
Beta vulgaris Petroleum ether 35 ± 10 4.7 ± 1.9 −2.4 ± 17.4 4.4 ± 14.8 −15.9 ± 29.4 44 ± 7 1.1 ± 0.3
Dichloromethane 33 ± 11 3.2 ± 2 −2.3 ± 5.5 43.7 ± 8.6 −20.2 ± 30.9 30 ± 5 1.1 ± 0.1
Cimicifuga americana Petroleum ether 37 ± 8 1.6 ± 0.7 −5.8 ± 14.4 27.1 ± 27.5 −3.7 ± 37 47 ± 10 1.4 ± 0.3
Dichloromethane 31 ± 5 2.8 ± 1.1 −16.2 ± 14.9 79.2 ± 19.5 5.8 ± 32.1 21 ± 9 1.4 ± 0.6
Cimicifuga racemosa DCM extract 34 ± 7 6.6 ± 3.2 −2.5 ± 1.3 3.5 ± 8.7 8.3 ± 5.2 45 ± 3 1.1 ± 0.4
75% EtOH 21 ± 5 5.8 ± 1.8 4.3 ± 5.3 −48.4 ± 4.9 0.1 ± 5.7 23 ± 3 1 ± 0.2
PE extract 55 ± 8 3.2 ± 1.5 −4.5 ± 4.8 21.3 ± 7.2 4.8 ± 10.9 64 ± 8 1 ± 0.5
Cimicifuga rubifolia Petroleum ether 56 ± 2 2.5 ± 2 −13.1 ± 12.4 33.2 ± 17 −23.2 ± 25 66 ± 6 1.1 ± 0.5
Dichloromethane 51 ± 5 7.4 ± 1.1 0.3 ± 15.9 21 ± 14.2 4.4 ± 19.8 40 ± 8 0.8 ± 0.3
Cornus officinalis Petroleum ether 26 ± 16 24.3 ± 6.6 2.6 ± 5.9 41.8 ± 4.1 11.5 ± 3.4 48 ± 4 1.5 ± 0.5
Dichloromethane 0 ± 4 2.5 ± 0.7 −2.2 ± 4.8 24.9 ± 5.2 30.6 ± 19.1 0 ± 2 1.3 ± 0.3
Daucus carota Petroleum ether 21 ± 6 12.3 ± 5.1 −6.8 ± 11.2 −26.6 ± 14.6 −18.7 ± 19.1 36 ± 6 0.9 ± 0.5
Dichloromethane 22 ± 5 5.4 ± 1.8 −5.7 ± 8 −11.5 ± 16.1 13.7 ± 14.1 26 ± 6 0.7 ± 0.2
Paeonia moutan Petroleum ether 21 ± 5 2.8 ± 1 −9.7 ± 11.4 8.7 ± 16.7 7.7 ± 19.7 60 ± 6 2 ± 1.7
Polygonum multiflorum Petroleum ether 15 ± 8 21.7 ± 8.5 6.5 ± 1.8 1.2 ± 3.3 7.6 ± 14.3 50 ± 9 1.4 ± 0.7
Dichloromethane 30 ± 7 25.8 ± 11.2 12.3 ± 3.4 0.4 ± 12.3 12.7 ± 17.8 36 ± 13 3 ± 2.5
Pueraria lobata Petroleum ether 32 ± 2 10.3 ± 2.8 −10.8 ± 12.6 46.9 ± 18.9 12.7 ± 30 36 ± 2 1.5 ± 1.4
Dichloromethane 63 ± 6 24.5 ± 9.9 −0.3 ± 25.6 36 ± 32.6 −3.3 ± 5.5 45 ± 19 1.2 ± 0.5
75% ethanol 17 ± 16 26.9 ± 12.7 −1.3 ± 13.8 28.9 ± 40.7 7.6 ± 50.1 63 ± 3 3.5 ± 1.3
Pueraria mirifica Petroleum ether 12 ± 8 6 N.T. −2.7 ± 2.6 58.5 ± 37 100.6 ± 38.8 18 ± 12 N.T.
Dichloromethane 12 ± 5 25.7 ± 9.5 −4.2 ± 1.1 101.1 ± 8.6 115 ± 2.2 19 ± 11 3.2 ± 1.6
75% ethanol 18 ± 7 28 ± 8.6 −1.4 ± 4.8 52.2 ± 61 60.4 ± 62.2 31 ± 8 2.4 ± 1.3
Valeriana officinalis Petroleum ether 43 ± 11 7.2 ± 3.1 −7.8 ± 7.6 33.7 ± 16.8 12.5 ± 19.3 81 ± 4 1.3 ± 0.8
Dichloromethane 13 ± 12 5.9 ± 1.6 −8.5 ± 7.7 19.4 ± 8.2 −7.6 ± 21 13 ± 6 0.7 ± 0.1
Viburnum prunifolium Dichloromethane 3 ± 6 0.7 ± 0.5 −3.5 ± 3 68.5 ± 6.3 42 ± 4.4 3 ± 4 1 ± 0
Vitex agnus-castus Petroleum ether 37 ± 6 9.5 ± 2.5 −1.1 ± 1.6 43.5 ± 3.3 39.1 ± 8.3 68 ± 9 1.1 ± 0.7
Dichloromethane 13 ± 3 9.6 ± 6.6 −2.3 ± 1.5 94.5 ± 7.2 112.5 ± 8.8 20 ± 19 0.8 ± 0.3
1

Percent binding at 200 μg/mL.

2

Fold induction where DMSO is 1.

3

Tested at 20 μg/mL.

4

Percent induction (estrogenic), inhibition (antiestrogenic), or cytotoxic.

5

Bold face-type indicates extract with assay activity.

6

Not tested due to the limited quantities available.