Fig. 8.

PMN lysis after phagocytosis requires new gene transcription and protein synthesis. a Actinomycin D (Act. D) or puromycin were combined with PMNs 30 min before addition of LAC and PMN lysis was measured 6 h after addition of S. aureus. b Actinomycin D or puromycin were combined with PMNs 30 min before addition of LAC (0 h), or at indicated times after addition of LAC, and PMN lysis was measured at 6 h. * p < 0.05 vs. PMNs + LAC in the absence of actinomycin D or puromycin (black bar, LAC alone); ^§ p < 0.05 vs. PMNs + LAC + actinomycin D or puromycin at t = 0 h using a one-way ANOVA and Dunnett's post-test (6 h). Results are the mean ± standard deviation of 4-6 (a) or 5-12 (b) PMN donors.