Figure 2.

Determining the requirement of Mps1 nuclear entry. (A) Schematic map of Mps1 mutants. The sequence elements that are responsible for Mps1 nuclear localization include two LXXLL motifs. (B) Representative images of YFP-tagged wild-type Mps1 and Mps1 mutants after incubation with 9 µM RO-3306 for 19 h. Nuclei were identified by DAPI staining. (C) Immunoblot analysis of the expression of ecotopically expressed YFP-tagged wild-type and mutant Mps1 along with the endogenous Mps1 using an anti-Mps1 (Millipore) raised against the N terminus of Mps1. (D) Quantification of Mps1 nuclear translocation upon RO-3306 treatment. About 50–70 cells for each Mps1 mutant were counted and quantified using the scheme described in Figure S1.