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The region including two LXXLL motifs is not involved in CRM1-dependent nuclear export of Mps1. (A) YFP-tagged wild-type and mutant Mps1 loss two LXXLL motifs (M1) or phosphorylation at S821 are treated with DMSO or Leptomycin B (200 ng/ml) for 2 h prior to imaging. (B) Quantitation of subcellular localization of wild-type, LXXLL deletion and S821 Mps1 upon treatment with Leptomycin B.
