Table 1.
Target gene | ORF | Description | fold change | adj p-value |
---|---|---|---|---|
ERO1 | PIPA00063 | Thiol oxidase required for oxidative protein folding in the endoplasmic reticulum | 6.49 | 9.15E-07 |
NCE103 * | PIPA03864 | Carbonic anhydrase, involved in non-classical protein export pathway | 3.36 | 4.08E-08 |
AQR1 * | PIPA04502 | Plasma membrane multidrug transporter of the major facilitator superfamily | 17.03 | 2.25E-09 |
SLY41 | PIPA02527 | Protein involved in ER-to-Golgi transport | 1.85 | 3.22E-04 |
TDH3 | PIPA02510 | Glyceraldehyde-3-phosphate dehydrogenase, involved in glycolysis and gluconeogenesis | 4.38 | 5.44E-06 |
TEF4 | PIPA00834, PIPA10574 | Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA to ribosomes | 36.25 | 6.15E-10 |
TSA1 | PIPA04168 | Thioredoxin peroxidase, self-associates to form a high-molecular weight chaperone complex under oxidative stress | 5.78 | 2.83E-06 |
WSC4 | PIPA00592 | ER membrane protein involved in translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane | 2.57 | 8.82E-05 |
Target genes (systematic names and P. pastoris specific PIPA codes) for co-expression are listed together with their biological function. The fold change ratios and adjusted p-values derive from the pairwise comparison of hypoxic and normoxic conditions. Target genes highlighted with (*) are also significantly (p-value < 0.05) upregulated in the Fab expressing strain compared to the control strain in hypoxic conditions.