Table 1.
Antibiotic intestinal decontamination (ATB) effect on stress-induced inflammatory, anti-inflammatory and oxidative/nitrosative parameters in control and CMS-exposed rats.
| Control | CMS | Control+ATB | CMS+ATB | |
|---|---|---|---|---|
| Plasma determinations | ||||
| LPS (EU/mL) | 0.2856 ± 0.027 | 0.3546 ± 0.006** | 0.248 ± 0.022 | 0.3008 ± 0.016# |
| LBP (ng/mL) | 799.8 ± 39.75 | 955.6 ± 35.57* | 840.0 ± 19.52 | 804.2 ± 32.97# |
| Brain determinations | ||||
| TLR-4 (mRNA) | 96.84 ± 2.618 | 109.8 ± 3.285** | 102.5 ± 2.703 | 101.0 ± 1.278 |
| TLR-4 (OD) (protein) | 99.26 ± 4.455 | 116.9 ± 3.093** | 88.09 ± 4.142 | 97.01 ± 3.162## |
| MD-2 (mRNA) | 98.01 ± 2.575 | 108.4 ± 2.178** | 91.74 ± 2.432 | 96.86 ± 3.912# |
| MD-2 (OD) (protein) | 94.94 ± 2.977 | 108.6 ± 2.578* | 102.6 ± 2.842 | 104.9 ± 4.381 |
| NF-κB p65 Activity (% Control) |
100.0 ± 4.571 | 85.48 ± 3.277* | 96.73 ± 15,33 | 71.66 ± 3.1** |
| NF-κB p65 (mRNA) | 101.8 ± 2.546 | 94.21 ± 2.193* | 90.28 ± 2.052 | 88.16 ± 2.879 |
| NF-κB p65 (OD) (protein) | 100.6 ± 3.363 | 87.23 ± 3.554* | 103.2 ± 4.530 | 99.43 ± 3.442# |
| IκBα (mRNA) | 100.0 ± 4.286 | 118.7 ± 6.436* | 95.55 ± 3.265 | 99.42 ± 5.101# |
| COX-2 (mRNA) | 99.89 ± 5.056 | 137.2 ± 8.159** | 124.6 ± 7.084 | 107.1 ± 6.181# |
| PGE2 (pg/mg prot.) |
45.14 ± 6.485 | 78.69 ± 12.24* | 48.58 ± 8.973 | 36.75 ± 7.877# |
| 15d-PGJ2 (pg/mg prot.) |
83.45 ± 13.99 | 42.00 ± 6.775* | 83.28 ± 13.78 | 107.8 ± 21.68# |
| IL-1β (mRNA) | 94.59 ± 4.000 | 114.0 ± 2.318** | 95.91 ± 9.424 | 91.35 ± 3.886## |
| MDA (nmol/mg prot.) |
0.00279 ± 0.000256 | 0.00372 ± 0.000285* | 0.00187 ± 0.000142 | 0.00242 ± 0.000344## |
Data are means ± SEM of 8-10 rats per group; * p < 0.05; ** p < 0.01 vs. Control; # p < 0.05; ## p < 0.01 vs. CMS. One-way ANOVA followed by the Newman-Keuls post hoc test.