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. 2011 Oct 18;11:95. doi: 10.1186/1472-6750-11-95

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Copyright ©2011 Burleigh et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Glutamine and glutamate metabolic profiles in batch shake flasks. The extracellular concentration of glutamine with 0 mM, 4 mM and 8 mM glutamine supplementation and ammonium (identical symbols, colored grey) in batch shake flask cultivation (A). The rate of glutamine consumption and ammonium production with varied glutamine concentrations in batch shake flask cultivation (symbols identical to previous, B). The extracellular concentration and rate of consumption of glutamate during batch shake flask cultivation (identical symbols, C and D respectively). Panels A, B, C and D, n = 6.