Table 2.

Glutamine controls recHCG glycoforms in bioreactor continuous culture.

Peak No.	Glycan Structure	Steady State 1 (8 mM Gln)	Steady State 2 (0 mM Gln)	Steady State 3 (8 mM Gln)	Steady State 4 (0 mM Gln)
1	A1	2.16 ± 0.66	4.57 ± 2.64	4.58 ± 2.05	4.44 ± 1.34
2	A2	1.13 ± 0.27	1.30 ± 0.21	2.31 ± 0.50	1.36 ± 0.24
3	A1G1	1.32 ± 0.24	1.90 ± 0.40	2.64 ± 0.60	5.89 ± 0.97
4	M5	4.56 ± 0.44	4.72 ± 0.61	6.77 ± 1.01	14.70 ± 3.37
5	A2G1, A1G1S1	3.60 ± 0.19	3.45 ± 0.06	3.81 ± 0.35	4.66 ± 0.81
6	A2G1S1	1.42 ± 0.19	1.63 ± 0.24	1.65 ± 0.22	2.41 ± 0.22
7	FA2G1	3.53 ± 0.47	3.54 ± 0.23	3.78 ± 0.42	2.40 ± 0.27
8	A2G2, FA2G1S1	7.39 ± 0.59	8.38 ± 0.91	9.29 ± 1.31	14.33 ± 2.08
9	FA2G2, A2G2S1	18.31 ± 0.98	18.03 ± 0.84	16.56 ± 0.36	22.06 ± 2.92
10	A2G2S2, FA2G2S1	26.90 ± 0.73	24.51 ± 1.04	20.90 ± 2.18	14.55 ± 1.15
11	A3G2S1, FA2G2S2	20.99 ± 1.60	20.14 ± 1.42	16.02 ± 2.29	8.87 ± 0.84
12	A3G2S2, A3G3S1	2.95 ± 0.23	2.79 ± 0.26	5.54 ± 1.28	2.23 ± 0.18
13	FA3G3S1, A3G3S2	2.67 ± 0.25	2.40 ± 0.19	3.77 ± 0.62	1.23 ± 0.17
14	A3G3S3, FA3G3S2	1.97 ± 0.11	1.70 ± 0.15	1.50 ± 0.29	0.57 ± 0.06
15	FA3G3S3	1.07 ± 0.14	0.96 ± 0.13	0.90 ± 0.14	0.30 ± 0.04

The relative structures and proportions of each peak of the HCG N-linked glycan NP-HPLC pool with varying concentrations of glutamine at each steady state of continuous culture are shown. Numbers in bold indicates significant difference, where each steady state was compared side-by-side, p < 0.05 and n = 3.