Figure 2. Classes of translated pseudogenes.

Peptides (light grey arrows) are shown to map within pseudogene boundaries (dary grey arrows), proving that the region is translated to protein. A, peptides in a single coherent open reading frame spanning the entire length of the pseudogene LIC 12015 from Leptospira interrogans. It is unclear why such regions were designated as pseudogenes. B, peptides fall within two different translation frames which are located within Geobacter sulfurreducens pseudogene GSU3073. This situation can arise when the underlying genome sequence is erroneous and contains artificial indels, or if the two regions are separately translated. C, peptides fall in a single open reading frame which only partially covers the genomic regions annotated as a pseudogene, Desulfovibrio vulgaris pseudogene DVU0699. This confirms the conceptual translation of the pseudogene (i.e. ending in an in-frame non-sense codon).