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. 2011 Nov 17;6(11):e27520. doi: 10.1371/journal.pone.0027520

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A) Cartoon depiction of the AAV inverted terminal repeat. The experimental oligonucleotides are also depicted as AA' (80-mer), A (39-mer) and A' (39-mer). B) hESCs were injected with AA' or a control oligonucleotide with no AAV sequence under the indicated conditions. The DNA solution contained rhodamine allowing visualization of injected cells (red). Microscopy was performed at the indicated time points. C) DNA microinjections of the A or A' oligonucleotides as described above. D) Native gel analysis of the A and A' oligonucleotides as described in the text.