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. 2011 Nov 17;6(11):e27767. doi: 10.1371/journal.pone.0027767

Figure 4. Effect of decreased K8 Ser 73 or Ser431 phosphorylation on migratory ability of OSCC cells.

Figure 4

(A) Representative fluorescence and phase contrast micrographs of K8 wild type (K8WT-1) and K8 phospho-mutants (K8S73A-1 or K8S431A-2) cells that displayed GFP-tagged K8 analyzed by fluorescence microscopy. Note that the cells expressing high levels of K8-Ser73Ala or K8-Ser431Ala mutant appeared on the edges of the colony. Scale bar: 50 µm. (B) Scratch wound healing assays were performed on K8 wild type (K8WT-1), K8-Ser73Ala (K8S73A-1) and K8-Ser431Ala (K8S431A-2) clones. Phase contrast images (10X) of wound closure at 0 hr (panels a–c), 2.5 hr (panels d–f), 5 hr (panels g–i), 7.5 hr (panels j–l), and 10 hr (panels m–o) of the clones are shown in the figure. Scale bar: 100 µm. Migration rate was calculated by AxioVision software. The data shown is the average from three independent experiments with the mean and standard deviation. *P<0.05, ** P<0.01 (by student t-test).