Table 3.
qRT-PCR probe and reaction descriptions
| Target group | Probe name target | Example of regression curve | r2 | PCR efficiency (%) | Acceptable data (%) |
|---|---|---|---|---|---|
| Methanosarcina | msar | y = -0.2547x +11.34 | 0.997 | 80 | 75 |
| Methanobacteriaceae | mrtA | y = -0.2691x+12.21 | 0.995 | 86 | 4 |
| Methanocorpusculaceae | mcp | y = -0.2627x+12.38 | 0.987 | 83 | 88 |
| Methanosaetaceae | msa | y = -0.2380x+10.27 | 0.943 | 73 | 52 |
There is a standard reference curve only for the Methanosarcina and Methanobatecteriaceae, making it possible to establish the gene copies in the extracted DNA. The last column indicates the percentage of determinable sample on the total 25 tested samples.