Skip to main content
. 2011 Nov 17;6(11):e27828. doi: 10.1371/journal.pone.0027828

Figure 6. Differential modulation of PGN mediated gene expression by MDP co-stimulation.

Figure 6

(A) ELISA for IL10, TNFα and RANTES. Normal of Nod2 knockout or scrambled sequence siRNA treated macrophages were stimulated for 12 hours with various ligands as indicated. Culture supernatant was checked for the presence of indicated cytokines. Results are presented as the mean of triplicate wells ± SD. Data is plotted on split semi-log graph. Statistical significance was checked by two way ANOVA. P value was found to be significant (<0.0001). (B) Western blot analysis of iNOS and COX2. Macrophages were stimulated for 6 hours as indicated and whole cell lysates were checked for the presence of iNOS and COX2 proteins. Results correspond to one representative experiment of three independent experiments.