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. 2011 Nov 17;6(11):e27879. doi: 10.1371/journal.pone.0027879

Figure 1. Characterization of Ran transgenic mice.

Figure 1

A, Tail DNA was extracted from transgenic mouse lines expressing Ran-WT, Ran T24N or Ran G19V in pancreatic β cells under RIP control, and PCR products were amplified using RIP-HA (top) or RIP-Ran (bottom) primers. (+) or (−) refers to Ran-positive or –negative genotype, or positive or negative control primers for the amplification reaction. M, molecular weight markers. B, Pancreas tissues extracted from the various mouse cohorts genotyped for the presence (+) or absence (−) of Ran transgenes were analyzed by Western blotting. 3T3, extracts from NIH3T3 cells transiently transfected with HA-Ran cDNA used as control. a-HA, antibody to HA. C: Pancreas or liver tissues were isolated from PCR-confirmed Ran transgenic mice, and analyzed by Western blotting. β-actin was used as a loading control.