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. 2011 Nov 17;6(11):e27879. doi: 10.1371/journal.pone.0027879

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Xia et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A and B, The indicated PCR-confirmed transgenic mice were analyzed for blood glucose content at 2 mo of age under ad libitum feeding (A) or fasting (B) conditions. Non-TG, non-transgenic mice. Glucose concentrations (mg/dl) in each group in A (number of mice in parentheses), and statistical analyses (unpaired t test) are as follows: Non-TG (n = 24), 125±2; Ran-WT (n = 26), 189.4±17.8, p = 0.013; Ran-G19V (n = 24), 153.7±6.5, p = 0.003; Ran-T24N (n = 21), 172.4±21.1, p = 0.02. Statistical data re-analysis of the groups in A using ANOVA and post-hoc multiple tests with Bonferroni procedure was as follows: Ran-WT, p<0.0001; Ran-G19V, 0.017; Ran-T24N, p = 0.029. C, The indicated non-TG or Ran transgenic mice were analyzed for blood insulin concentrations. Insulin levels (ng/ml) in each group (number of mice in parentheses), and statistical analyses (unpaired t test) are as follows: Non-TG (n = 8), 1.26±0.1; Ran-WT (n = 11), 0.7±0.16, p = 0.019; Ran-G19V (n = 5), 0.61±0.03, p = 0.0008; Ran-T24N (n = 7), 0.49±0.14, p = 0.0009. Statistical data re-analysis using ANOVA and post-hoc multiple tests with Bonferroni procedure was as follows: Ran-WT, p = 0.023; Ran-G19V, p = 0.031; Ran-T24N, p = 0.004. One outlier mouse in the Ran-G19V group with aberrantly high insulin level (2.35 ng/ml) was excluded from the analysis. For panels A–C, each point corresponds to an individual mouse. D, Islets (20/well) isolated from non-TG or Ran-WT transgenic mice were incubated with 5 mM D-glucose, and analyzed for insulin release in the supernatant at the indicated time intervals. Mean±SD of replicates. E, Islets (20/well) isolated from non-TG (black) or Ran-WT (purple), Ran-G19V (grey) or Ran-T24N (blue) transgenic mice were incubated with 16.7 mM glucose, and analyzed for insulin release in the supernatant at the indicated time intervals. Mean±SD of replicates.