Figure 6. Copurification of Sho and misfolded PrP from ScN2a-Sho-1 cell lysates.

Sho copurified with PrP molecules in lysates prepared from ScN2a-Sho-1 cells, but not with PrP^C in N2a-Sho cells, as demonstrated by coimmunoprecipitation analyses. Nonspecific binding of misfolded PrP to the immunoprecipitation matrix was assessed by performing immunoprecipitations on ScN2a cells that do not express Sho and on ScN2a-Sho-1 cells in the absence of antibody (Mock). Samples were not treated with PK prior to Western blotting. Sho and PrP were probed using antibodies 06rSH-1 and HuM-D18, respectively. Molecular masses based on the migration of protein standards are shown in kilodaltons.