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. 2011 Nov 17;7(11):e1002382. doi: 10.1371/journal.ppat.1002382

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Watts et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Levels of Sho were decreased in RML prion-infected Tg(MoSho) mice compared to uninfected controls. For comparison, Sho and protease-resistant PrP^Sc levels in RML-infected, wt FVB mice are shown. The antibody HuM-P was used to probe PrP, and the antibody 06rSH-1 used to detect Sho. Actin levels are shown as a control. (B) RML prion infection resulted in decreased levels of both full-length and endoproteolytically trimmed Sho in Tg(MoSho) mice. All samples were treated with PNGaseF. The 06SH-3a antibody recognizing a C-terminal Sho epitope was used. (C) Quantification of Sho levels in wt and Tg(MoSho) mice (n = 3 for each group) following infection with RML prions. In all infected mice, Sho levels decreased by ∼70% compared to uninfected mice. (D) Levels of Sho were decreased in RML prion-infected Tg(HuSho) mice compared to uninfected controls. The antibody HuM-P was used to probe PrP, and the antibody S-12 used to detect Sho. Actin levels are shown as a control. For all Western blots, molecular masses based on the migration of protein standards are shown in kilodaltons.