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. 1985 Sep 25;13(18):6499–6513. doi: 10.1093/nar/13.18.6499

Human immunoglobulin kappa light chain genes of subgroups II and III.

H G Klobeck, A Meindl, G Combriato, A Solomon, H G Zachau
PMCID: PMC321973  PMID: 2997711

Abstract

The first complete sequences of functionally rearranged VK genes (abbreviations ref. 1) of subgroups II and III are reported. The genes have been cloned from lymphoid cell lines synthesizing KII or KIII light chains as evidenced from immunochemical analyses with anti-VK subgroup-specific antisera. These data, together with the sequence of a KIV gene (described in the accompanying paper) and those of previously published KI genes make possible a comparison of genes representative of the four known V region subgroups of human K light chains. The VKII gene is distinguished from the VKI, VKIII, and VKIV genes by a much longer intron within the leader sequence: 426 bp vs ca. 120-220 bp. Blot hybridization experiments with human DNA digests using probes from the KII and KIII genes and from the respective upstream regions help to define subgroup specific probes and hybridization conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentley D. L. Most kappa immunoglobulin mRNA in human lymphocytes is homologous to a small family of germ-line V genes. Nature. 1984 Jan 5;307(5946):77–80. doi: 10.1038/307077a0. [DOI] [PubMed] [Google Scholar]
  2. Bentley D. L., Rabbitts T. H. Evolution of immunoglobulin V genes: evidence indicating that recently duplicated human V kappa sequences have diverged by gene conversion. Cell. 1983 Jan;32(1):181–189. doi: 10.1016/0092-8674(83)90508-1. [DOI] [PubMed] [Google Scholar]
  3. Bentley D. L., Rabbitts T. H. Human V kappa immunoglobulin gene number: implications for the origin of antibody diversity. Cell. 1981 Jun;24(3):613–623. doi: 10.1016/0092-8674(81)90088-x. [DOI] [PubMed] [Google Scholar]
  4. Bentley D. L., Rabbitts T. H. Human immunoglobulin variable region genes--DNA sequences of two V kappa genes and a pseudogene. Nature. 1980 Dec 25;288(5792):730–733. doi: 10.1038/288730a0. [DOI] [PubMed] [Google Scholar]
  5. Bornkamm G. W., Kaduk B., Kachel G., Schneider U., Fresen K. O., Schwanitz G., Hermanek P. Epstein-Barr virus-positive Burkitt's lymphoma in a German woman during pregnancy. Blut. 1980 Mar;40(3):167–177. doi: 10.1007/BF01008574. [DOI] [PubMed] [Google Scholar]
  6. Breathnach R., Chambon P. Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981;50:349–383. doi: 10.1146/annurev.bi.50.070181.002025. [DOI] [PubMed] [Google Scholar]
  7. Falkner F. G., Zachau H. G. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements. Nature. 1984 Jul 5;310(5972):71–74. doi: 10.1038/310071a0. [DOI] [PubMed] [Google Scholar]
  8. Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
  9. Garoff H., Ansorge W. Improvements of DNA sequencing gels. Anal Biochem. 1981 Aug;115(2):450–457. doi: 10.1016/0003-2697(81)90031-2. [DOI] [PubMed] [Google Scholar]
  10. Hieter P. A., Max E. E., Seidman J. G., Maizel J. V., Jr, Leder P. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell. 1980 Nov;22(1 Pt 1):197–207. doi: 10.1016/0092-8674(80)90168-3. [DOI] [PubMed] [Google Scholar]
  11. Honjo T. Immunoglobulin genes. Annu Rev Immunol. 1983;1:499–528. doi: 10.1146/annurev.iy.01.040183.002435. [DOI] [PubMed] [Google Scholar]
  12. Jaenichen H. R., Pech M., Lindenmaier W., Wildgruber N., Zachau H. G. Composite human VK genes and a model of their evolution. Nucleic Acids Res. 1984 Jul 11;12(13):5249–5263. doi: 10.1093/nar/12.13.5249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Khoury G., Gruss P. Enhancer elements. Cell. 1983 Jun;33(2):313–314. doi: 10.1016/0092-8674(83)90410-5. [DOI] [PubMed] [Google Scholar]
  14. Klobeck H. G., Combriato G., Zachau H. G. Immunoglobulin genes of the kappa light chain type from two human lymphoid cell lines are closely related. Nucleic Acids Res. 1984 Sep 25;12(18):6995–7006. doi: 10.1093/nar/12.18.6995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Klobeck H. G., Solomon A., Zachau H. G. Contribution of human V kappa II germ-line genes to light-chain diversity. Nature. 1984 May 3;309(5963):73–76. doi: 10.1038/309073a0. [DOI] [PubMed] [Google Scholar]
  16. Ledford D. K., Goñi F., Pizzolato M., Franklin E. C., Solomon A., Frangione B. Preferential association of kappa IIIb light chains with monoclonal human IgM kappa autoantibodies. J Immunol. 1983 Sep;131(3):1322–1325. [PubMed] [Google Scholar]
  17. Lenoir G. M., Preud'homme J. L., Bernheim A., Berger R. Correlation between immunoglobulin light chain expression and variant translocation in Burkitt's lymphoma. Nature. 1982 Jul 29;298(5873):474–476. doi: 10.1038/298474a0. [DOI] [PubMed] [Google Scholar]
  18. Levy J. A., Virolainen M., Defendi V. Human lymphoblastoid lines from lymph node and spleen. Cancer. 1968 Sep;22(3):517–524. doi: 10.1002/1097-0142(196809)22:3<517::aid-cncr2820220305>3.0.co;2-a. [DOI] [PubMed] [Google Scholar]
  19. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  20. Nilsson K., Sundström C. Establishment and characteristics of two unique cell lines from patients with lymphosarcoma. Int J Cancer. 1974 Jun 15;13(6):808–823. doi: 10.1002/ijc.2910130609. [DOI] [PubMed] [Google Scholar]
  21. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983 Dec;26(1):101–106. doi: 10.1016/0378-1119(83)90040-9. [DOI] [PubMed] [Google Scholar]
  22. Parslow T. G., Blair D. L., Murphy W. J., Granner D. K. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence. Proc Natl Acad Sci U S A. 1984 May;81(9):2650–2654. doi: 10.1073/pnas.81.9.2650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pech M., Jaenichen H. R., Pohlenz H. D., Neumaier P. S., Klobeck H. G., Zachau H. G. Organization and evolution of a gene cluster for human immunoglobulin variable regions of the kappa type. J Mol Biol. 1984 Jun 25;176(2):189–204. doi: 10.1016/0022-2836(84)90420-0. [DOI] [PubMed] [Google Scholar]
  24. Pech M., Zachau H. G. Immunoglobulin genes of different subgroups are interdigitated within the VK locus. Nucleic Acids Res. 1984 Dec 21;12(24):9229–9236. doi: 10.1093/nar/12.24.9229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rimm D. L., Horness D., Kucera J., Blattner F. R. Construction of coliphage lambda Charon vectors with BamHI cloning sites. Gene. 1980 Dec;12(3-4):301–309. doi: 10.1016/0378-1119(80)90113-4. [DOI] [PubMed] [Google Scholar]
  26. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  27. Stavnezer J., Kekish O., Batter D., Grenier J., Balazs I., Henderson E., Zegers B. J. Aberrant recombination events in B cell lines derived from a kappa-deficient human. Nucleic Acids Res. 1985 May 24;13(10):3495–3514. doi: 10.1093/nar/13.10.3495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tanigaki N., Yagi Y., Moore G. E., Pressman D. Immunoglobulin production in human leukemia cell lines. J Immunol. 1966 Nov;97(5):634–646. [PubMed] [Google Scholar]
  29. Tonegawa S. Somatic generation of antibody diversity. Nature. 1983 Apr 14;302(5909):575–581. doi: 10.1038/302575a0. [DOI] [PubMed] [Google Scholar]
  30. Zachau H. G., Pech M., Klobeck H. G., Pohlenz H. D., Straubinger B., Falkner F. G. Wie entstehen die Antikörper? 9. Fritz-Lipmann-Vorlesung. Hoppe Seylers Z Physiol Chem. 1984 Dec;365(12):1363–1373. [PubMed] [Google Scholar]

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