Table 1.
Selected candidate reference genes, primers, and PCR reactions efficiencies
| Gene name | GeneBank accession number | Primer sequence (forward/reverse) | Amplicon length (bp) | Amplification efficiency (%) | R2 | Average Ct of cDNA | ||
|---|---|---|---|---|---|---|---|---|
| 1 Day | 2 months | 5 months | ||||||
| B2M | NM_213978.1 | ACTTTTCACACCGCTCCAGT CGGATGGAACCCAGATACAT | 180 | 86.83 | 0.999 | 20.23 | 19.24 | 20.63 |
| BLM | NM_001123084.1 | TCCTCACCTTCTGCATTTCC GTGGTGGCTGAGAATCCTGT | 152 | 95.94 | 0.995 | 25.29 | 24.12 | 24.89 |
| GAPDH | AF017079.1 | ACCCAGAAGACTGTGGATGG ACGCCTGCTTCACCACCTTC | 247 | 95.95 | 0.991 | 26.82 | 26.22 | 26.29 |
| HPRT1 | NM_001032376.2 | AACCTTGCTTTCCTTGGTCA TCAAGGGCATAGCCTACCAC | 150 | 81.88 | 0.997 | 22.27 | 21.28 | 22.29 |
| PPIA | NM_214353.1 | CACAAACGGTTCCCAGTTTT TGTCCACAGTCAGCAATGGT | 171 | 82.96 | 0.995 | 16.82 | 16.31 | 17.61 |
| RPL4 | DQ845176.1 | AGGAGGCTGTTCTGCTTCTG TCCAGGGATGTTTCTGAAGG | 185 | 91.07 | 0.995 | 16.65 | 16.80 | 17.32 |
| SDHA | DQ178128.1 | AGAGCCTCAAGTTCGGGAAG CAGGAGATCCAAGGCAAAAT | 149 | 86.41 | 0.989 | 20.55 | 20.64 | 22.34 |
| TBP | DQ178129.1 | ACGTTCGGTTTAGGTTGCAG GCAGCACAGTACGAGCAACT | 118 | 99.59 | 0.995 | 24.44 | 23.92 | 24.31 |
| YWHAZ | DQ178130.1 | ATTGGGTCTGGCCCTTAACT GCGTGCTGTCTTTGTATGACTC | 146 | 93.83 | 0.997 | 20.35 | 19.64 | 19.92 |
*R2, correlation coefficient of the slope of the standard curve