Table 2.
Expression stability of nine candidate reference gens evaluated by BestKeeper software
| B2M | BLM | GAPDH | HPRT1 | PPIA | RPL4 | SDHA | TBP | YWHAZ | BK | |
|---|---|---|---|---|---|---|---|---|---|---|
| Irrespective of age | ||||||||||
| n* | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 |
| SD [± Ct] | 1.91 | 1.36 | 1.56 | 2.12 | 1.69 | 1.55 | 1.90 | 1.19 | 1.56 | 1.49 |
| CV [% Ct] | 9.54 | 5.50 | 5.90 | 9.67 | 9.99 | 9.16 | 8.95 | 4.92 | 7.81 | 7.07 |
| 1day | ||||||||||
| n** | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 |
| SD [± Ct] | 1.86 | 1.70 | 1.42 | 2.11 | 1.70 | 1.69 | 1.61 | 1.30 | 1.99 | 1.47 |
| CV [% Ct] | 9.17 | 6.70 | 5.28 | 9.45 | 10.11 | 10.17 | 7.82 | 5.30 | 9.76 | 6.95 |
| 2momths | ||||||||||
| n** | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 |
| SD [± Ct] | 1.37 | 1.10 | 1.19 | 1.73 | 1.35 | 1.05 | 1.49 | 1.04 | 0.96 | 1.11 |
| CV [% Ct] | 7.13 | 4.55 | 4.54 | 8.02 | 8.30 | 6.24 | 7.23 | 4.36 | 4.89 | 5.38 |
| 5 months | ||||||||||
| n** | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 | 42 |
| SD [± Ct] | 2.49 | 1.47 | 2.01 | 2.86 | 2.04 | 2.00 | 2.21 | 1.31 | 1.92 | 1.92 |
| CV [% Ct] | 12.07 | 5.92 | 7.65 | 13.12 | 11.56 | 11.57 | 9.89 | 5.39 | 9.64 | 8.97 |
Descriptive statistics of nine candidate reference genes based on their cycle threshold (Ct) values. In the last column the BestKeeper (BK) index is computed together with descriptive parameters for the nine genes. Abbreviations: CV [%Ct]: the coefficient of variance expressed as a percentage on the Ct level; SD [± Ct]: the standard deviation of the Ct; Results from overall tissues irrespective of age and in different ages (1 day, 2 months and 5 months) are shown. * indicates the number of samples (since BestKeeper tool has limitation for 100 samples, the average Ct for three individuals was used for analysis); ** indicates the average for triplicate run was used for analysis.