Figure 1.

Examples of sequencing errors identified by proteogenomic analysis. (A) The nucleotide and corresponding amino acid sequences of the fusA gene. The 'G' at genome position 3, 440, 920 was previously erroneously recognized as 'T', resulting in a stop codon mutation. (B) The nucleotide and corresponding amino acid sequences of the zwf gene and its pseudogene. An extra 'A' at genome position 1, 899, 437 resulted in a frameshift that caused a premature termination mutation. These two sequencing errors were corrected in GenBank entries on our request. Unambiguously assigned peptides and sequencing error bases are boxed. *, stop codon.