Table 2.
Characteristics of seven novel ORFs
| Gene tag | Strand | Length (AA) |
Overlapsa) | Annotation in other enterobacteria |
|---|---|---|---|---|
| BIO01608b) | + | 80 | No | Hypothetical protein |
| BIO50043b) | - | 365 | Partial (S) | Sulfate/thiosulfate transporter subunit |
| BIO07235b) | + | 25 | Partial (S) | None |
| BIO43803b) | - | 496 | Partial (C) | Hypothetical protein |
| BIO68373 | - | 59 | Nested (C) | Conserved hypothetical protein |
| BIO58539 | - | 86 | Nested (S) | None |
| BIO48527 | - | 36 | Nested (S) | None |
a) No, ORFs not overlapping other genes; Partial (C), ORFs partially overlapping known genes on the complementary strand; Partial (S), ORFs partially overlapping known genes on the same strand; Nested (C), ORFs completely contained within known genes on the complementary strand; Nested (S), ORFs completely contained within known genes on the same strand, but in a different frame.
b) The transcripts of novel ORFs were confirmed by RT-PCR assay.