Table 1.
Strains and Vectors Used in this Study
| Strain/tdlasmid/Cosmid | Relevant Characteristics | Source |
|---|---|---|
| Strain | ||
| Escherichia coli DH5α | deoR, endA1, gyrA96, hsdR17(rK– mK+), recA1 phoA, relA1, thi-1, Δ(lac ZYA-argF) U169φ80dlacZΔM15, F–, λ–, supE44 | Takara Bioa |
| Plasmid/Cosmidb | ||
| pSTV28 | Low-copy-number cloning vector, Cmr | Takara Bio |
| pSTV::pfaE | pSTV28 carrying pfaE from S.pneumatophoruse SCRC-2738 | [7] |
| pNEB139 | High-copy-number expression vector, Ampr | New England Biolabsc |
| pEPAΔ1,3,4,9 | pNEB carrying pfaA–E from S.pneumatophori SCRC-2738 | [9] |
| Stratagened | ||
| pWE15 | Cosmid expression vector, Kmr, Ampr | Takara Bio |
| pEPAΔ1,2,3 | pWE15 carrying an EPA gene cluster that lacks pfaE from S. pneumatophori SCRC-2738 | [3] |
a
Takara Bio Inc., Tokyo, Japan.
b
Abbreviations of antibiotics: Km, kanamycin; Amp, ampicillin; and Cm, chloramphenicol.
c
New England Biolabs, Ipswich, MA, USA.
d
Stratagene, La Jolla, CA, USA.