Table 2.
Effects of Various Hydrophilic and Hydrophobic Compounds on the Growth of Cells of E. coli DH5α Recombinants with EPA (DH5αEPA+) and without EPA (DH5αEPA–)
| MICs of Various Compoundsa | |||
|---|---|---|---|
| Solvent | DH5αEPA+ | DH5αEPA– | |
| Reactive oxygen species (MW) | |||
| H2O2 (34.0) | Water | 100 µM | 10 µM |
| tert-BHP (90.1) | Water | 100 µM | 10 µM |
| Radical generator | |||
| AAPH (271.19) | Water | 10 µM | 1 µM |
| AMVN (248.37) | 1% Ethanolb | 1 µM | 10 µM |
| Antibiotics | |||
| Ampicillin sodium (371.4) | Water | >500 µg/ml | 500 µg/ml |
| Kanamycin sulfate (582.6) | Water | >500 µg/ml | 500 µg/ml |
| Streptomycin sulfate (1457.4) | Water | 3 µg/ml | 0.3 µg/ml |
| Oxidative phosphorylation uncouplers | |||
| CCCP (204.1) | 1% Ethanol | 1 mM | 10 mM |
a
MW, molecular weight; MICs, minimal inhibitory concentrations; AAPH, 2,2΄-azobis-(2-amidopropane)hydrochloride; AMVN, 2,2΄-azobis-(2,4-dimethyl)valeronitrile tert-BHP, tert-butyl hydroxyl peroxide; CCCP, carbonyl cyanide m-chloro phenyl hydrazone.
b
Final concentration.