Table 3.
Effects of Various Concentrations of Ethanol on the Growth of Cells of E. coli DH5α Recombinants Producing Various Levels of EPA
| OD600 of Cultures Containing Ethanol at Approximatelya | |||||
|---|---|---|---|---|---|
| 0% | 3% | 5% | 6% | 7% | |
| E. coli DH5α cellsb carrying | |||||
| pEPAΔ1,3,4,9 | 3.4 ± 0.1 | 3.1 ± 0.4 | 1.5 ± 0.1 | 0.03 ± 0.01 | NGc |
| pEPAΔ1,2,3 + pSTV::pfaEd | 3.4 ± 0.2 | 2.9 ± 0.4 | 1.0 ± 0.1 | 0.01 ± 0.00 | NG |
| pEPAΔ1,2,3 + pSTVd | 3.2 ± 0.2 | 2.8 ± 0.4 | 0.4 ± 0.1 | NG | NG |
a
Values are average ± standard deviation of three independent measurements.
b
Cells were grown for 7 days at 20 °C.
c
No growth detected.
d
pEPAΔ1,2,3 + pSTV::pfaE and pEPAΔ1,2,3 + pSTV are DH5αEPA+ and DH5αEPA–, respectively.