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asnO gene expression RT-PCR analysis of RNAs isolated from S. meliloti GMI211 wild-type strain (wt) or GMI5704 fixJ mutant strain (fixJ) grown in minimal medium M9 in either oxic (+) or microoxic (2% O2) conditions (-). RT-PCR were performed with either asnO or hemA specific primers and the products were separated on agarose gels, blotted on a nylon membrane and hybridized with the corresponding 32P labelled PCR product and analysed on a Phosphorimager.
