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. Author manuscript; available in PMC: 2012 Jun 2.
Published in final edited form as: Neuroscience. 2011 Mar 30;183:230–237. doi: 10.1016/j.neuroscience.2011.03.035

Figure 3.

Figure 3

Lysophosphatidylcholine (LPC)-induced focal demyelination of the sciatic nerve induced a substantial increase in the frequency of micturition. The micturition frequency was monitored for each animal (2.5 hours) before and after random assignment to one of three treatment conditions. Micturition frequency was significantly different from pre-operative averages from 7–28 days in animals subjected to a combination of cholera toxin β subunit conjugated to a fluorescent tracer (CTB-555) injected into the wall of the bladder and LPC-induced focal nerve demyelination. Control conditions including animals subjected to either bladder wall injections of CTB-555 only or sham-injury in combination with CTB555 did not reach significance by day 7. All treatment conditions were subjected to bladder wall injections of CTB-555 at least 7 days prior to micturition frequency assays. Analysis was performed using two-way ANOVA followed by the Bonferroni post-hoc pairwise comparisons (*p < 0.01).