FIG. 2.

Expression of main gluconeogenic enzymes in the kidneys (A–C) and the intestine (D–F) of fed L-G6pc^−/− mice. A and D: Level of G6pc and Pck1 mRNA expressed as a ratio relative to the Rpl19 mRNA level. B and E: Western blot and quantification analysis for G6PC and PEPCK-C proteins. Actin is shown as a loading control. C and F: Specific G6Pase and PEPCK-c activity of homozygous L-G6pc^−/− (black bar) and WT (white bar) mice. Data were obtained 5 weeks after gene deletion in fed mice (n = 6 mice per group) and are expressed as the mean ± SEM. Values significantly different from WT (*P < 0.05, **P < 0.01) are indicated. AU, arbitrary units.