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. 2011 Nov 13;60(12):3235–3245. doi: 10.2337/db11-0616

FIG. 1.

FIG. 1.

Knockdown of SirT1 mimicking that seen in obesity causes anorexia-driven weight loss and TNF-α elevation. A–C: Body weight (***P < 0.001) (n = 8–12/group) (A), anorexia (n = 12/group) (B), and loss of epididymal fat mass (n = 3–4/group) (C) of ad libitum fed rats treated with ASO biweekly for 1 month. D: Results from parallel measurement of plasma appetite regulatory hormones in animals treated with control or SirT1 ASO showing elevated TNF-α and possibly IL-6 in the SirT1 group (n = 7–8/group, pooled). E: Plasma TNF-α levels (n = 5/group). F: Adipose tissue levels of TNF-α (n = 3–4/group). G: SirT1 protein expression in WAT from animals treated with control ASO and fed normal chow, animals treated with SirT1 ASO and fed normal chow, and mildly diabetic animals fed high-fat/high-fructose chow. H: Quantification of representative data presented in F (n = 6/group). I: Plasma TNF-α levels in normal chow–fed control ASO–treated rats, normal chow–fed SirT1 ASO–treated rats, and high-fat diet–fed, control ASO–treated rats by electrochemiluminescence (n = 4–5/group). HFD, high-fat diet.