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. 2011 Nov 13;60(12):3208–3216. doi: 10.2337/db10-1192

FIG. 3.

FIG. 3.

Regulation of Pet1 in the pancreas. A: The mRNA encoding Pet1 (Fev) was amplified by RT-PCR from RNA isolated from mPAC cells infected with adenovirus expressing β-galactosidase (Adeno-LacZ) or human neurogenin3 (Adeno-NEUROG3) and from mouse E15.5 embryonic pancreas. B: The relative expression levels of Fev mRNA were quantified by real-time PCR from RNA isolated from mPAC cells infected with adenovirus expressing the proteins shown. C–E: The relative expression levels of Fev mRNA were quantified by real-time PCR with pancreas RNA isolated at E18.5 from mouse embryos with targeted deletions of Neurog3 (C), Nkx2.2 (D), and Nkx6.1 (E). All data points represent means ± SEM of at least three independent experiments. *P < 0.05 vs. heterozygote and vs. wild type (D); **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. cells infected with adenovirus expressing β-galactosidase (B); and ****P < 0.0001 vs. wild type (C) by two-tailed Student t test. Numerical values of expression level relative to control are shown above lowest expressing samples. Und., undetected. In situ hybridization studies for Fev with immunofluorescent staining for Nkx2.2 and Neurog3 are shown in Supplementary Fig. 4.