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. 2011 Nov 13;60(12):3110–3120. doi: 10.2337/db11-0061

FIG. 2.

FIG. 2.

Glucose represses Gck expression concomitantly with induction of G6pc and Pklr. Hepatocytes were precultured for 18 h in medium containing 5 mmol/L glucose and then incubated for 4 h in fresh MEM without (hatched line) or with 10 nmol/L insulin and 5 mmol/L (5) or 25 mmol/L glucose (25) without or with 2 μmol/L S4048 [(25)S]. A: Glucose phosphorylation (GP, nmol/h mg protein) and glucose 6-P (G6P, nmol/mg protein). B: mRNA levels of the target genes indicated expressed relative to 5 mmol/L glucose without insulin (first bar). Means ± SEM, n = 3–6. *P < 0.05, **P < 0.01, ***P < 0.005, effect of insulin; #P < 0.05, ##P < 0.01, ###P < 0.005, effect of substrate; ^P < 0.05, ^^P < 0.01, ^^^P < 0.005, effect of S4048. ND, not determined.