Table 2.
Effects of PMN-activation on selectin function at 2 dyne/cm2
| Adhesion [PMN/mm2] | |||
|---|---|---|---|
| Blocking antibody | HUVEC++/PMN- | HUVEC++/PMN++ | HUVEC++/PMN++/PLT++ |
| NONE | 1042 ± 61 | 591 ± 43 | 1313 ± 25 |
| L- | 744 ± 67 * | 607 ± 56 ns vs NONE | Ø |
| P- | 833 ± 59 ns vs NONE | 596 ± 85 ns vs NONE | 396 ± 35 * vs NONE |
| E- | 504 ± 55 * vs NONE | 267 ± 32 * vs NONE | Ø |
| L-/P- | 674 ± 48 ns vs L- | Ø | Ø |
| E-/P- | 504 ± 91 | 230 ± 12 ns vs E- | Ø |
| L-/E- | 405 ± 59 * vs L- | Ø | Ø |
| L-/E-/P- | 343 ± 40 | Ø | Ø |
Adhesion in lipopolysaccharide-activated cultures (100 ng/ml; HUVEC++, PMN++, PLT++) at 2 dyne/cm2. Ø (not determined); * and ns (P < 0.05 versus indicated group or not significant, respectively). Statistical analysis with paired t-tests and correction after Bonferroni-Holm (mean ± SEM; n = 4).
For comparison, background adhesion in non-activated cultures (HUVEC-/PMN-) at 2 dyne/cm2 revealed 247 ± 52 PMN/mm2.
HUVEC, human umbilical venous endothelial cells; PMN, polymorphonuclear neutrophils; PLT, platelets; L-, leukocyte selectin; P-, platelet selectin; E-, endothelial selectin; SEM, standard error of the mean.