Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Mol Genet Metab. 2011 Nov 1;104(3):338–345. doi: 10.1016/j.ymgme.2011.05.021

Figure 2. PTC124 mediates translational read through of PPT1-nonsense mutations.

Figure 2

(A) PPT1-myc-FLAG construct used to transfect COS-1 cells is shown schematically. Myc and FLAG tags are incorporated at the C-terminus of PPT1. A point mutation is designed within the PPT1 sequence that causes generation of truncated PPT1-protein without FLAG or myc tags. However translational read through mediated by PTC124 can produce full length PPT1-protein with FLAG and myc tags. (B) Immunofluorescence analysis of PTC124 (5 µg/ml) treated and untreated COS-1 cells transfected with nonsense PPT1-myc-FLAG using FLAG antibody. Note that some PTC124-treated cells showed immunorectivity for FLAG, but no immunoreactivity for FLAG was observed in untreated cells.