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. 2011 Nov 10;7(11):e1002226. doi: 10.1371/journal.pcbi.1002226

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Narayanan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Recombinant Arp2/3 complex WT (rWT) and R105/106A ARPC4 expressed and purified from sf21 cells. (b) Pyrene actin assembly assays comparing nucleation activity of rWT and R105/106A ARPC4 rArp2/3 complex. (c) Pointed end capping assay with actin alone (black), rWT (red) and R105/106A ARPC4 rArp2/3 (green), and R105/106A ARPC4 rArp2/3 pretreated with Antarctic phosphatase (AP-R105/106A ARPC4) (purple).