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. Author manuscript; available in PMC: 2011 Nov 18.
Published in final edited form as: Biochemistry. 2011 Jul 21;50(33):7218–7227. doi: 10.1021/bi200853y

Figure 5.

Figure 5

DMSO increases the stability of myosin-IIA filaments. Myosin-IIA assembly was monitored as a function of salt concentration (millimolar) using a standard sedimentation assay. (A) Coomassie-stained SDS-PAGE showing representative reaction mixtures (m) and supernatants (s) from assembly assays performed in the absence or presence of 2% DMSO. (B) Myosin-IIA assembly assay. The amount of myosin-IIA recovered in the supernatant was determined by densitometry of Coomassie-stained gels. Values represent the mean ± standard deviation for three independent experiments.