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. Author manuscript; available in PMC: 2011 Nov 18.
Published in final edited form as: Biochemistry. 2011 Jul 21;50(33):7218–7227. doi: 10.1021/bi200853y

Figure 6.

Figure 6

Mutation of C81 disrupts S100A4-mediated disassembly of myosin-IIA filaments. Coomassie-stained SDS-PAGE showing representative reaction mixtures (m) and supernatants (s) from disassembly assays performed with wild-type and substituted S100A4 proteins (C81A, C81S, C3R/C86S) in the absence (A) or presence of 2% DMSO (B). Substitution of C81 with either alanine or serine inhibited S100A4-mediated depolymerization of myosin-IIA filaments, which was more pronounced in the presence of DMSO. (C) Quantification of disassembly assays performed at 1:1 and 5:1 S100A4 dimers:myosin-IIA rod. Values represent the mean ± standard deviation for 2−5 independent experiments.