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. 2011 Dec;52(12):2136–2147. doi: 10.1194/jlr.M017566

Fig. 2.

Fig. 2.

Expression of mCherry-tagged seipin proteins in HeLa cells. A: Schematic diagrams of seipin-expressing pmCherry-N1 and pmCherry-C1 plasmids. Seipin has one short isoform (seipinS) which encodes 398 amino acids and one long isoform (seipinL), which encodes 462 amino acids. B: HeLa cells were transiently transfected with a plasmid expressing SEC61B-GFP and plasmids expressing either seipinL-mCherry, seipinS-mCherry, mCherry-seipinL, or mCherry-seipinS, and observed for GFP and mCherry signal. Bar, 10 μm. C: Immunoblot analysis of mCherry, mCherry-tagged wild-type and mutant seipin proteins. Arrow head, mCherry-tagged short isoform of seipin; double arrow head, mCherry-tagged long isoform of seipin. D: Lysates prepared from HeLa cells expressing either seipinL-mCherry, seipinS-mCherry, or a mutant of seipinS-mCherry, S90L, were treated with or without endo H and probed with mCherry antibody.