Fig. 4.

Expression of PAT family members in human MCs. Transcript levels of the PAT family members plin1, plin2, plin3, plin4, and plin5 were determined by RT-PCR using mRNA from human MCs at 14 wk of culture (MC, lane 1), LAD2 cells (LAD2, lane 2), and sets of sequence-specific primers. Human reference total RNA was used as positive control (pCtrl, lane 3), and PCR performed without the addition of template served as negative control (nCtrl, lane 4). Beta actin-specific primers were used to test the quality of cDNA templates (bottom panel). The PCR products were analyzed by agarose gel electrophoresis.