Fig. 5.

Lipidation of apoA-I is reduced when EL and SAA are coexpressed in hepatoctyes. Mice were injected with a total of 6 × 10¹⁰ particles per mouse, consisting of a mixture of AdNull, AdEL (1 × 10¹⁰ particles), AdSAA (5 × 10¹⁰ particles), or AdEL + AdSAA as indicated. Primary hepatocyte cultures were prepared 24 h after infusion, treated with the LXR agonist T0901317 for 8 h to upregulate ABCA1, and then incubated for 18 h with 15 μg/ml lipid-free apoA-I. A: Culture supernatants were separated by nondenaturing GGE and immunoblotted using anti-human apoA-I. Lipid-free apoA-I was included on the gel for comparison. B: Lysates of cells prepared before or after T0901317 treatment (10 µg cell protein) were separated on a 4-20% polyacrylamide gradient gel and immunoblotted using anti-ABCA1.